A humanized anti-M2 scFv shows protective in vitro activity against influenza
Identifieur interne : 001146 ( Main/Exploration ); précédent : 001145; suivant : 001147A humanized anti-M2 scFv shows protective in vitro activity against influenza
Auteurs : J. Gabbard [États-Unis] ; N. Velappan [États-Unis] ; R. Di Niro [Norvège] ; J. Schmidt [États-Unis] ; C. A. Jones [États-Unis] ; S. M. Tompkins [États-Unis] ; A. R. M. Bradbury [États-Unis]Source :
- Protein Engineering, Design & Selection [ 1741-0126 ] ; 2009.
Abstract
M2 is one of the most conserved influenza proteins, and has been widely prospected as a potential universal vaccine target, with protection predominantly mediated by antibodies. In this paper we describe the creation of a humanized single chain Fv from 14C2, a potent monoclonal antibody against M2. We show that the humanized scFv demonstrates similar activity to the parental mAb: it is able to recognize M2 in its native context on cell surfaces and is able to show protective in vitro activity against influenza, and so represents a potential lead antibody candidate for universal prophylactic or therapeutic intervention in influenza.
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DOI: 10.1093/protein/gzn070
Affiliations:
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Gabbard</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Infectious Diseases, Animal Health Research Center, Influenza Pathogenesis and Immunology Research Center, College of Veterinary Medicine, University of Georgia, 111 Carlton Street, Building 1077, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Velappan, N" sort="Velappan, N" uniqKey="Velappan N" first="N." last="Velappan">N. Velappan</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>B Division, Los Alamos National Laboratory, TA-43, HRL-1, Los Alamos, NM 87545</wicri:regionArea><placeName><region type="state">Nouveau-Mexique</region></placeName></affiliation></author><author><name sortKey="Di Niro, R" sort="Di Niro, R" uniqKey="Di Niro R" first="R." last="Di Niro">R. Di Niro</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Centre for Immune Regulation, Institute of Immunology, Rikshospitalet, University of Oslo, Sognsvannsveien 20, N-0027 Oslo</wicri:regionArea><wicri:noRegion>N-0027 Oslo</wicri:noRegion></affiliation></author><author><name sortKey="Schmidt, J" sort="Schmidt, J" uniqKey="Schmidt J" first="J." last="Schmidt">J. Schmidt</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>B Division, Los Alamos National Laboratory, TA-43, HRL-1, Los Alamos, NM 87545</wicri:regionArea><placeName><region type="state">Nouveau-Mexique</region></placeName></affiliation></author><author><name sortKey="Jones, C A" sort="Jones, C A" uniqKey="Jones C" first="C. A." last="Jones">C. A. 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In this paper we describe the creation of a humanized single chain Fv from 14C2, a potent monoclonal antibody against M2. We show that the humanized scFv demonstrates similar activity to the parental mAb: it is able to recognize M2 in its native context on cell surfaces and is able to show protective in vitro activity against influenza, and so represents a potential lead antibody candidate for universal prophylactic or therapeutic intervention in influenza.</div></front></TEI><affiliations><list><country><li>Norvège</li><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li><li>Nouveau-Mexique</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Gabbard, J" sort="Gabbard, J" uniqKey="Gabbard J" first="J." last="Gabbard">J. Gabbard</name></region><name sortKey="Bradbury, A R M" sort="Bradbury, A R M" uniqKey="Bradbury A" first="A. R. M." last="Bradbury">A. R. M. 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